目的 考察单味药白芍、白芍-当归药对、复方四物汤中芍药苷在体肠吸收机制的影响规律。方法 采用在体循环灌流法,应用HPLC测定肠吸收循环液中芍药苷质量浓度,UV测定肠吸收循环液中即时酚红质量浓度。分别从pH值、药物浓度、吸收部位、P-gp 4方面考察芍药苷吸收情况。结果 通过对单味药、药对及复方中的芍药苷吸收情况进行比较,发现相同吸收部位、pH值、浓度(以芍药苷浓度为准)的样品溶液中,复方四物汤组芍药苷的吸收量、Ka、药物的累积吸收百分率较其他组显著增大(P<0.05),同时t1/2显著减小(P<0.05)。白芍、药对及复方样品液与P-gp的抑制剂和诱导剂合用时,芍药苷的吸收表现出吸收量、Ka、药物的累积吸收百分率较对照组均有显著的增加或减少(P<0.05),同时t1/2也显著性减少或增加(P<0.05)。结论 芍药苷的吸收受P-gp的影响,在相同条件下复方中芍药苷的吸收较药对及单味药中的芍药苷吸收情况更佳。
Abstract
OBJECTIVE To investigate the absorption mechanism of paeoniflorin in Radix Paeoniae Alba, both of Radix Paeoniae Alba and Radix Angelica Sinensis and Siwu Decoction. METHODS The circulatory perfusion technique was used in this study, the concentrations of paeoniflorin and phenol red in intestinal absorption circulation were respectively determined by HPLC and UV. The effects of pH value, drug concentration, absorption site and P-gp on the absorption of paeoniflorin were investigated separately. RESULTS By comparing the absorption of paeoniflorin in Radix Paeoniae Alba, both of Radix Paeoniae Alba and Radix Angelica Sinensis, and Siwu Decoction, it was found that in the sample solution at the same absorption site, pH value and concentration (based on the concentration of paeoniflorin), the absorption, Ka and cumulative absorption of paeoniflorin in compound Siwu decoction group were significantly increased compared with the other groups (P<0.05), while t1/2 was significantly decreased (P<0.05). When combined with the inhibitor and inducer of P-gp, the absorption of paeoniflorin showed significant increase or decrease in the amount of absorption, Ka and cumulative absorption% compared with the control group (P<0.05), and t1/2 also showed significant decrease or increase (P<0.05). CONCLUSION The absorption of paeoniflorin could be affected by P-gp, and under the same conditions, the absorption of paeoniflorin in complicated Chinese herbal formula is better than that in the single herb and herb-pair.
关键词
芍药苷 /
白芍 /
药对 /
四物汤 /
在体肠吸收
{{custom_keyword}} /
Key words
peoniflorin /
Radix Paeoniae Alba /
herb-pair /
Siwu decoction /
in situ intestinal absorption
{{custom_keyword}} /
中图分类号：
R965
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] ZHENG Z J. Formula of Traditional Chinese Medicine(方剂学)[M]. Vol 1. Shanghai:Shanghai Scientific & Technical Publishers, 2008:139.
[2] FAN X S, CHEN F, LIU P, et al. Analogous formulae of Si-wu decoction for nourishing blood and regulating menstruation[J]. Word Sci Technol Mod Tradit Chin Med(世界科学技术-中医药现代化), 2013, 15(2):177-182.
[3] GAO Y, MA Z C, LIANG Q D, et al. Si-Wu-Tang and blood deficiency[J]. Word Sci Technol Mod Tradit Chin Med(世界科学技术-中医药现代化), 2010, 12(2):211-216.
[4] ZHANG T R. Modern research progress of Siwu Tang[J]. Beijing J Tradit Chin Med(北京中医药), 2012, 31(1):73-76.
[5] ZHAO X H, ZHENG M D, LONG Q, et al. HPCE determination of ferulic acid in Siwu Decoction[J]. Hebei Med J(河北医药), 2012, 34(13):2049-2050.
[6] WANG Y H, WEI Y Q, WANG W, et al. Determination of paeoniflorin in Siwu Decoction by RP-HPLC[J]. Chin Tradit Pat Med(中成药), 2005, 28(8):910-913.
[7] ZHENG Y B, XIAO G C, TONG S L, et al. Paeoni florin inhibits human gastric carcinoma cell proliferation through up-regulation of micro RNA-124 and suppression of PI3K/Akt and STAT3 signaling [J]. World J Gastroenterol, 2015, 21(23):7197-7202.
[8] ZHANG Q, YUAN Y, CUI J, et al. Paeoniflorin inhibits pro-liferation and invasion of breast cancer cells through sup-pressing Notch-1 signaling pathway [J]. Biomed Pharmacother, 2016, 1(19):197-203.
[9] NAM K N, YAE C G, HONG J W, et al. Paeoniflorin, a monoterpene glycoside, attenuates lipopolysaccharide-in-duced neuronal injury and brain microglial inflammatory re-sponse[J]. Biotechnol Lett, 2013, 35(8):1183-1189.
[10] WANG K, ZHU L, ZHU X, et al. Protective effect of paeoni-florin on Aβ25-35-induced SH-SY5Y cell injury by prevent-ing mitochondrial dysfunction [J]. Cell Mol Neurobiol, 2014, 34 (2):227-234.
[11] WU T, ZHENG J, ZHANG C L, et al. Advances in studies on intestinal absorption of traditional Chinese medicine[J]. Drug Eval(药品评价), 2013, 10(6):30-35.
[12] ZHU R H, ZHAO J N, BI Y Q, et al. Advances in studies on intestinal absorption kinetics of Chinese materia medica[J]. Drug Eval Res(药物评价研究), 2010, 33(1):25-29.
[13] FOX C B, KIM J, LE L V, et al. Micro/nanofabricated platforms for oral drug delivery[J]. J Controlled Release, 2015, 219(33):431-444.
[14] DUAN J A, SU S L, TANG Y P, et al. Modern understanding of compatible combinations of traditional Chinese medicine[J]. J Nanjing Univ Chin Med(南京中医药大学学报), 2009, 25(5):330-333.
[15] LI X R, ZHAO J, LAN Z G, et al. Analysis of volatile oil in herbal pair Chuanxiong Rhizome-Notopterygium Root by gas chromatography-mass spectrometry and chemometric resolution method[J]. J Cent South Univ(Sci Technol)(中南大学学报:自然科学版), 2007, 38(4):681-685.
[16] PANG T, MAI L Y, CHEN Y, et al. Research progress on chemical constituents of traditional Chinese medicine for compatibility[J]. J Chin Med Mater(中药材), 2015, 38(11):2429-2434.
[17] LI J, FU Y, GAO Y Y. New progress in the study of the allocation of effective groups of traditional Chinese medicine[J]. Chin J Inform Tradit Chin Med(中医药信息), 2014, 31(3):162-164.
[18] AN R, ZHANG H, ZHANG Y Z, et al. Intestinal absorption of different combinations of active compounds from Gegenqinlian decoction by rat single pass intestinal perfusion in situ[J]. Acta Pharm Sin(药学学报), 2012, 47(12):1696-1702.
[19] LIANG X L, LIAO Z G, ZHU J Y, et al. The absorption characterization effects and mechanism of Radix Angelicae dahuricae extracts on baicalin in Radix Scutellariae using in vivo and in vitro absorption models[J]. J Ethnopharmacol, 2011,139(1):1-57.
[20] BAI J, DING Z D, XU C R, et al. Study on in situ intestinal absorption of ferulic acid in herbs couples of Radix Angelica Sinensis and Gusticum Chuanxiong Hort[J]. J Harbin Univ Com(Nat Sci Ed)(哈尔滨商业大学学报:自然科学版), 2013, 29(2):129-132.
[21] LI W L, NAN L L, JI Y B, et al. Studies on influence factors of ginsenoside Rg1 and Rb1 absorption in intestines of rats[J]. China J Chin Mater Med(中国中药杂志), 2009, 34(20):2627-2632.
[22] CHEN Y, WANG J Y, YUAN L, et al. Interaction of the main com-ponents from the traditional Chinese drug pair Chaihu-Shaoyao based on rat intestinal absorption[J]. Molecules, 2011, 16(11):9600-9609.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
哈尔滨商业大学校级科研项目资助(18XN083)
{{custom_fund}}
PDF(5344 KB)
